Device
Part:BBa_K2325101:Design
Designed by: Wenyong Lou Group: iGEM17_SCUT-FSE-CHINA (2017-10-16)
Formamidase expression device
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 189
Illegal NgoMIV site found at 676
Illegal NgoMIV site found at 871
Illegal NgoMIV site found at 1018 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
Mutagenesis to remove internal PstI site of the formamidase gene.
Source
the formamidase gene is cloned from the genome of Paenibacillus pasadenensis.